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高雄醫學大學醫學院臨床醫學研究所

黃阿梅老師實驗室

 

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實驗室介紹

研究重點
本實驗室主要探討轉錄因子CCAAT/enhancer binding protein delta (C/EBPdelta, C/EBPδ, CEBPD)在正常與癌症細胞中對不同環境因子及內生性壓力所參與的訊息傳遞路徑之調控,以進而瞭解C/EBPdelta所扮演之角色及作用機轉。
研究方向
本實驗室以轉錄因子CCAAT/enhancer binding protein delta (C/EBPdelta, C/EBPδ, CEBPD)為主題研究其在
1.老鼠胚胎纖維母細胞(mouse embryonic fibroblasts, MEFs)中維護基因體完整性的機制探討;
2.細胞中誘導細胞凋亡及調節細胞週期的機制探討;
3人類膀胱泌尿上皮癌細胞株的抗藥性的功能及角色扮演;
4.治療癌細胞抗藥性的天然藥物開發及其機轉之探討。
期望能在未來將所得到的知識應用到人類疾病和癌症的預防、診斷及治療。

執行之專題研究計畫 (before 2012)

1. 計畫名稱:轉錄因子C/EBPdelta維護基因體完整性的角色之探討 (2006/1~2006/7)
計畫編號:Q095018 (高雄醫學大學補助新聘/升等教師專題研究計畫)
參與工作:主持人
2. 計畫名稱:C/EBPdelta蛋白質在乳癌中決定細胞凋亡的機制探討(2006/8~2007/7)
計畫編號:NSC 95-2320-B-037-048
參與工作:主持人
3. 計畫名稱:C/EBPdelta參與cisplatin抗藥性機轉之探討(2006/12~2007/7)
計畫編號:Q096009 (高雄醫學大學補助新聘/升等教師專題研究計畫)
參與工作:主持人
4. 計畫名稱:轉錄因子Cebpd在老鼠胚胎纖維母細胞中維護基因體完整性的分子機制探討
(2008/4/1~2008/12/31)
計畫編號:KMU-M097004 (高雄醫學大學補助教師專題研究計畫)
參與工作:主持人
5. 計畫名稱:從大型生物網路中識別乳癌化療敏感性與化療抗性調控網路之差異表現
(2009/8/1~2010/7/31)
計畫編號:NSC98-2218-E-231-002- (國科會)
參與工作:共同主持人
6. 計畫名稱:轉錄因子CEBPD參與在泌尿上皮細胞癌對cisplatin抗藥性的分子機制探討
(2008/8/1~2011/7/31) 三年期
計畫編號:NSC97-2320-B-037-018-MY3 (國科會)
參與工作:主持人


研究成果:會議壁報發表(posters)(click)

研究成果:專利 (click)
1.專利名稱: 熊果酸衍生物及其醫護組合物
發明人: 林忠男、涂皇堯、黃阿梅、魏百祿、顏金鳳、侯自銓、楊世群、蒲永孝、翁舷誌
證書號碼: I386415 (中華民國)
2.專利名稱: 作用於微小管抗癌藥物2',5'-dimethoxychalcone衍生物之合成與活性評估
發明人: 林忠男、涂皇堯、黃阿梅、侯自銓、楊世群、蒲永孝、張建國
專利證書號碼: I419687 (中華民國)
3.專利名稱: 抗人類泌尿上皮癌之香桂超臨界萃取物、製備方法及用途
發明人: 李志琚B黃阿梅、陳中一、連培榮、劉瓊惠
專利證書號碼: I448295 (中華民國)
4.專利名稱:The anti-human urothelial carcinoma of supercritical carbon dioxide extract of cinnamomum subavenium, and the preparation process and uses
發明人: 李志琚B黃阿梅、陳中一、連培榮、劉瓊惠
(Jih-Heng Li, A-Mei Huang, Chung-Yi Chen, Pei-Jung Lien, Chiung-Hui Liu)
專利證書號碼:US8,669,377B2 (美國)
5.專利名稱: 熊果酸衍生物及其醫護組合物
發明人: 林忠男、涂皇堯、黃阿梅、魏百祿、顏金鳳、侯自銓、楊世群、蒲永孝、翁舷誌
證書號碼: US 8729055B2 (美國)
6.專利名稱: 18β-甘草次酸衍生物及其用途
發明人: 林忠男、林凱偉、黃阿梅、侯自銓、楊世群、蒲永孝、張建國
證書號碼: I424839 (中華民國)
7.專利名稱: Derivative of 18β-glycyrrhetinic acid APT to suppress cancer cells
發明人: 林忠男、林凱偉、黃阿梅、侯自銓、楊世群、蒲永孝、張建國
證書號碼: US 8,686,178 B2 (美國)

研究成果:期刊論文發表 (2013前 1~28)

No.
Title
Journal
IF and ranking
1
Huang, AM, Chang, TJ, Cho, WL, and Chou, CK. 1998. From mosquito to man: identification of a novel protein kinase, HsHPK, which is highly expressed in human hepatoma tissues.(全文) J. Biomed. Sci. 1998. 5(2): 135-140. (SCI)

IF: 2.013
ranking: 45/82 (2008)

2
Lee, HS, Huang, AM, Huang, GT, Yang, PM, Chen, PJ, Sheu, JC, Lai, MY, Lee, SC, Chou, CK, and Chen, DS. 1998. Hepatocyte growth factor stimulates the growth and activates mitogen-activated protein kinase in human hepatoma cells. (全文) J. Biomed. Sci. 1998. 5(3): 180-184. (SCI) IF: 2.013
ranking: 45/82 (2008)
3
Shen, HH, Huang, AM, Hoheisel, J, and Tsai, SF. 2001. Identification and characterization of a SET/NAP protein encoded by a brain-specific gene, MB20. (全文) Genomics. 2001. 71(1): 21-33. (SCI) IF: 3.327
ranking: 37/152 (2009)
4
Liu, MA, Huang, AM, Chou, CK, Liaw, GJ, and Wu, CW. 2001. Utilization of Drosophila eye to probe the functions of two mammalian Serine/Threonine kinases, Snk and HsHPK. (全文) J. Biomed. Sci. 2001. 8(3): 270-277. (SCI) IF: 2.013
ranking: 45/82 (2008)
5
Huang, AM, Montagna, C, Ni, Y, Sharan, S, Ried, T and Sterneck, E. 2004. Loss of CCAAT/enhancer binding protein delta promotes chromosomal instability. (全文) Oncogene. 2004. 23(8): 1549-1557. (SCI) IF:7.216
ranking: 13/141
6
Thangaraju M, Rudelius, M, Bierie, B, Raffeld, M, Sharan, S, Hennighausen, L, Huang, AM, and Sterneck, E. 2005. C/EBP delta is a crucial regulator of pro-apoptotic gene expression during mammary gland involution. (全文) Development. 2005. 132(21):4675-4685. (SCI) IF: 6.812
ranking: 5/37
7
Huang, AM, Rudelius, M, Sharan, S, McAllister, JM, Raffeld, M, Christenson, LK, and Sterneck, E. 2007. The Cebpd (C/EBPdelta) gene is induced by luteinizing hormones in ovarian theca and interstitial cells but is not essential for mouse ovary function. (全文) PLoS ONE 2007. 2: e1334. (SCI) IF: 4.351
ranking: 9/73 (2009)
8
Lin, KW#, Huang, AM#, Tu, HY, Weng, JR, Hour, TC, Wei, BL, Yang, SC, Wang, JP, Pu, YS, and Lin, CN*. 2009. Phloroglucinols inhibit chemical mediators and xanthine oxidase, and protect cisplatin-induced cell death by reducing reactive oxygen species in normal human urothelial and bladder cancer cells. (全文)

J Agric Food Chem 2009. 57: 8782-8787. (SCI)
(#co-first author)

IF: 2.562
ranking: 2/35 (2008)
9
Tu, HY#, Huang, AM#, Wei, BL, Gan, KH, Hour, TC, Yang, SC, Pu, YS, and Lin, CN*. 2009. Ursolic acid derivatives induce cell cycle arrest and apoptosis in NTUB1 cells associated with reactive oxygen species. (全文) Bioorg Med Chem 2009. 17: 7265-7274. (SCI)
(#Co-first author)
IF: 3.075
ranking: 10/40 (2008)
10
Pu, YS, Huang, CY, Kuo, YZ, Kang, WY, Liu, GY, Huang, AM, Yu, HJ, Lai, MK, Huang, SP, Wu, WJ, Chiou, SJ, and Hour, TC*. 2009. Characterization of membranous and cytoplasmic EGFR expression in human normal renal cortex and renal cell carcinoma.(全文) J. Biomed. Sci. 2009. 16(1): 82-92. (SCI) IF: 2.013
ranking: 45/82 (2008)
11
Wang, YC, Tsai, YS, Huang, JL, Lee, KW, Kuo, CC, Wang, CS, Huang, AM, Chang, JG, Jong, YJ and Lin, CS*. 2010. Arecoline arrests cells at prometaphase by deregulating mitotic spindle assembly and activating spindle assembly checkpoint. (全文) Oral Oncology. 2010. 46(4):255-62. (SCI) IF: 2.928
ranking: 5/55 (2009)
12
Chen, HL, Lin, KW, Huang, AM, Tu, HY, Wei, BL, Hour, TC, Yen, MH, Pu, Y and Lin, CN*. 2010. Terpenoids induce cell cycle arrest and apoptosis from the stems of Celastrus kusanoi associated with reactive oxygen species. (全文)  J Agric Food Chem 2010. 58(6):3808-12. (SCI) IF: 2.469
ranking: 2/44 (2009)
13
Tu HY, Huang AM, Hour TC, Yang SC, Pu YS, and Lin CN*. 2010. Synthesis and biological evaluation of 2',5'-dimethoxychalcone derivatives as microtubule-targeted anticancer agents. (全文) Bioorg Med Chem. 2010. 18 (6): 2089-2098. (SCI) IF: 2.822
ranking: 17/57 (2009)
14
Hour, TC, Lai, YL, Kuan, CI, Chou, CK, Wang, JM, Tu, HY, Hu, HT, Lin, CS, Wu, WJ, Pu, YS, Sterneck, E, and Huang, AM*. 2010. Transcriptional up-regulation of SOD1 by CEBPD: a potential target for cisplatin resistant human urothelial carcinoma cells. (全文) Biochem. Pharmacol. 2010. 80(3):325-334. (SCI) (*corresponding author) IF: 4.254
ranking: 34/237 (2009)
15
Pawar, SA, Sarkar, TR, Balamurugan, K, Sharan, S, Wang, J, Zhang, Y, Dowdy, SY, Huang, AM, and Sterneck E*. 2010. C/EBPdelta targets cyclin D1 for proteasome-mediated degradation via induction of CDC27/APC3 expression. (全文) Proc Natl Acad Sci USA. 2010. 107(20):9210-5. (SCI) IF: 9.432
ranking: 3/50 (2009)
16
Huang, CY, Chen, JYF, Wu,JE, Pu, YS, Liu, GY, Pan, MH, Huang, YT, Huang, AM, Hwang, CC, Chung, SJ, and Hour, TC*. 2010. Ling-Zhi polysaccharides potentiates cytotoxic effects of anti-cancer drugs against drug-resistant urothelial carcinoma cell.(全文) J Agric Food Chem. 2010. 58(15):8798-805. (SCI) IF: 2.469
ranking: 2/44 (2009)
17
Wang J, Sarkar TR, Zhou M, Sharan S, Ritt DA, Veenstra TD, Morrison DK, Huang, AM, and Sterneck E*. 2010. C/EBPdelta-mediated nuclear import of FANCD2 by IPO4 augments cellular response to DNA damage. (全文)  Proc Natl Acad Sci USA. 2010. 107(37):16131-6. (SCI) IF: 9.432
ranking: 3/50 (2009)
18
Lin CN#, Huang AM#, Lin KW#, Hour TC, Ko HH*, Yang SC, and Pu YS. 2010. Xanthine oxidase inhibitory terpenoids of Amentotaxus formosana protect cisplatin-induced cell death by reducing reactive oxygen species (ROS) in normal human urothelial and bladder cancer cells. (全文) Phytochemistry 2010. 71(17-18):2140-6. (SCI)
(#Co-first author)
IF: 3.104
ranking: 24/173 (2009)
19
Pan YC, Li CF, Ko CY, Pan MH, Chen PJ, Tseng JT, Wu WC, Chang WC, Huang AM, Sterneck E, and Wang JM*. 2010. CEBPD reverses RB/E2F1-mediated gene repression and participates in HMDB-induced apoptosis of cancer cells. (全文) Clin Cancer Res. 2010. 16(23):5770-80. (SCI) IF: 6.747
ranking: 16/166 (2009)
20
Lin, KW#, Huang, AM#, Tu, HY, Lee, LY, Wu, Hour, TC, Yang, SC, Pu, YS and Lin, CN*. 2011. Xanthine Oxidase Inhibitory Triterpenoid and Phloroglucinol from Guttiferaceous Plants Inhibit Growth and Induced Apoptosis in Human NTUB1 Cells Through a ROS-Dependent Mechanism. (全文) J Agric Food Chem. 2011. 59(1):407-14. (SCI) (#Co-first author) IF: 2.816
ranking: 2/55 (2010)
21
Ko, CY, Chang, LH, Lee, YC, Sterneck, E, Cheng, CP, Chen, SH, Huang, AM, Tseng, JT, and Wang, JM*. 2010. CAAT/enhancer binding protein delta (CEBPD) elevating PTX3 expression inhibits macrophage-mediated phagocytosis of dying neuron cells.(全文) Neurobiology of Aging. 2012. 33(2): 422.e11-422.e25 (SCI) IF: 6.189
ranking: 2/44 (2011)
22
Cheng,JH#, Huang, AM#, Hour, TC, Yang, SC*, Pu, YS and Lin, CN*. 2011. Antioxidant xanthone derivatives induce cell cycle arrest and apoptosis and enhance cell death induced by cisplatin in NTUB1 cells associated with ROS. (全文) Eur J Med Chem. 2011. 46(4):1222-31. (SCI) (#Co-first author) IF:3.193
ranking: 11/54 (2010)
23
Chao, SY, Chiang, JH*, Huang, AM*, and Chang, WS. 2011. An integrative approach to identifying cancer chemoresistance-associated pathways. (全文) BMC Med Genomics 2011. 4(1):23-37. (SCI)
(*co-correspondence)
IF:3.766
ranking: 47/156 (2010)
24
Huang, AM*, Kao, YT, Toh, S, Lin, PY, Chou, CH, Hu, HT, Lu, CY, Liou, JY, Chao, SY, Hour, TC and Pu, YS. 2011. UBE2M-mediated p27Kip1 degradation in gemcitabine cytotoxicity. (全文)
Biochem. Pharmacol. 2011. 82(1):35-42. (SCI) (*corresponding author) IF: 4.889
ranking: 20/249 (2010)
25
Liu, CH, Chen, CY, Huang, AM* and Li, JH*. 2011. Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1. (全文)

J Ethnopharmacology 2011. 137(1): 503-511 (SCI) (*co-correspondence)

IF:2.466
ranking: 37/187 (2010)
26
Lin, KW, Huang, AM, Hour, TC, Yang, SC*, Pu, YS and Lin, CN*. 2011. 18β-Glycyrrhetinic acid derivatives induced mitochondrial-mediated apoptosis through reactive oxygen species-mediated p53 activation in NTUB1 cells. (全文) Bioorg Med Chem
2011. 19(14):4274-85
 
IF:2.978
ranking: 15/54 (2010)
27
Wu, SR, Li, CF, Hung, LY, Huang, AM, Tseng, JT, Tsou, JH and Wang, JM.* 2011. CCAAT/enhancer binding protein delta mediates TNFalpha-induced aurora kinase C transcription and promotes genomic instability. (全文)

J. Biol. Chem. 2011.
286(33):28662-70

IF: 5.328 ranking: 50/286 (2010)
28
Tu, HY#, Huang, AM#, Teng, CH#, Hour, TC, Yang, SC*, Pu, YS and Lin, CN*. 2011. Anthraquinone derivatives induce G2/M cell cycle arrest and apoptosis in NTUB1 cells. (全文) Bioorg Med Chem. 2011. 19: 5670-5678 (#Co-first author) IF:2.978
ranking: 15/54 (2010)

Tel (O): 07-3121101 ext. 2138-17, Fax : 07-3223075, E-mail: amhuang@kmu.edu.tw
聯絡地址:高雄市(807)三民區十全一路100
高雄醫學大學醫學院臨床醫學研究所
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